Review



pcmv vsv g  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Addgene inc pcmv vsv g
    Pcmv Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pmc12907852-54-26-27
    Average 96 stars, based on 3107 article reviews
    pcmv vsv g - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Plasmid Preparation:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Expressing:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Transfection:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Next-Generation Sequencing:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Produced:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Titration:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Real-time Polymerase Chain Reaction:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Recombinant:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Modification:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Software:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS

    Virus:

    Article Title: Cell‐Type‐Specific Autophagy in Human Leukocytes
    Article Snippet: Western blotting was used to verify LC3B KO. psPAX2 was a gift from Didier Trono (Addgene plasmid #12260; http://n2t.net/addgene:12260 ; RRID:Addgene_12260). pCMV‐VSV‐G was a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454 ; RRID:Addgene_8454).

    Article Title: RalGAP complexes control secretion and primary cilia in pancreatic disease.
    Article Snippet: Next-generation sequencing and data analysis were performed by the Core Facility Genomics of the Medical Faculty, University Münster. pLKO.1 puro and pCMV-VSV-G plasmids were a gift from Bob Weinberg (Addgene plasmids # 8453 and #8454). psPAX2 plasmid was a gift from Didier Trono (Addgene plasmid # 12260).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEAR-luciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Transcription factor EB (TFEB) activity increases resistance of TNBC stem cells to metabolic stress.
    Article Snippet: The following plasmids were obtained from Addgene: psPAX2 (12260; Dr. Didier Trono), pCMV-VSV-G (8454; Dr. Bob Weinberg), pRK5-HA GST RagC 75L (19305; Dr. David Sabatini), and 4XCLEARluciferase reporter (66800; Dr. Albert La Spada).

    Article Title: Heat shock protein family D member 1 mediates lung cancer cell‑induced angiogenesis of endothelial cells
    Article Snippet: Using a third‐generation lentivirus system, lentiviral particles were produced by co‐transfecting 293T cells (CRL‐3216; ATCC) with either pLKO.1‐puro‐shHSPD1 (6 μg) or pLKO.1‐puro‐shControl (6 μg), along with packaging plasmids, including pMDLg/pRRE (a gift from Dr Didier Trono, Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland; Addgene plasmid no. 12251), pRSV‐Rev (a gift from Dr Didier Trono, Addgene no. 12253), and pCMV‐VSV‐G (a gift from Dr Bob Weinberg, Addgene plasmid no. 8454) using jetPRIME transfection reagent (Polyplus) for 12 h at 37 ̊C.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages
    Article Snippet: Lenti- virus envelope and packaging vector, pCMV- VSV- G and pCMV- dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages
    Article Snippet: Lenti-virus envelope and packaging vector, pCMV-VSV-G and pCMV-dR8.2 dvpr, respectively, were gifts from Prof. Bob Weinberg (Addgene plasmid #8454 and #8455). pOG44 (V600520) was obtained from Thermo Fisher Scientific.

    Article Title: Characterization and bioinformatic filtering of ambient gRNAs in single-cell CRISPR screens using CLEANSER.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER sgNT-73_gRNA_FW: CGGTGGAGTTTAAGAGCTATGCTG This paper Method Details See Table S6 for lentiviral titration qPCR primers This paper Method Details See Table S7 for individual gRNA oligos This paper Method Details Recombinant DNA pCMV-VSV-G Addgene Addgene Cat#8454 pRSV-Rev Addgene Addgene Cat#12259 pMDLg/pRRE Addgene Addgene Cat#12259 pJR85 Addgene Addgene Cat#140095 hU6 modified direct capture perturb-seq vector This paper Addgene Cat#230934 Software and algorithms Excel Microsoft https://www.microsoft.com/en-us/ microsoft-365/excel Rstudio Rstudio https://rstudio.com Biorender Biorender https://biorender.com/ CLEANSER This paper GitHub: https://github.com/ Gersbachlab-Bioinformatics/CLEANSER, Zenodo: https://doi.org/10.5281/zenodo.12744932 Cell Ranger Cell Ranger https://www.10xgenomics.com/support/ software/cell-ranger/latest Bowtie2 Bowtie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml OPEN ACCESS



    Similar Products

    86
    Cell Biolabs Inc pcmv vsv g
    Pcmv Vsv G, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/g+pcmv+vsv/bio_rxiv__64898__2026__04__30__722011-202-31-32
    Average 86 stars, based on 1 article reviews
    pcmv vsv g - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    96
    Addgene inc pcmv vsv g
    Pcmv Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pmc12907852-54-26-27
    Average 96 stars, based on 1 article reviews
    pcmv vsv g - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc barcoded lenti sgrna cre plasmids
    Barcoded Lenti Sgrna Cre Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pmc12907852-54-21-27
    Average 96 stars, based on 1 article reviews
    barcoded lenti sgrna cre plasmids - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc pcmv
    Pcmv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pmc13007490-36-21-22
    Average 96 stars, based on 1 article reviews
    pcmv - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc packaging plasmids pcmv vsv g
    Packaging Plasmids Pcmv Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41931390-226-21-24
    Average 96 stars, based on 1 article reviews
    packaging plasmids pcmv vsv g - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc vsv g envelope protein
    Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41922576-124-21-25
    Average 96 stars, based on 1 article reviews
    vsv g envelope protein - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc envelop plasmid
    Envelop Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41910395-246-17-23
    Average 96 stars, based on 1 article reviews
    envelop plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc pcmv vsv g stewart
    Pcmv Vsv G Stewart, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41915470-258-28-32
    Average 96 stars, based on 1 article reviews
    pcmv vsv g stewart - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc vsv g
    Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41911287-172-17-18
    Average 96 stars, based on 1 article reviews
    vsv g - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Addgene inc addgene plasmid
    Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv-vsv-g/pCMV-VSV-G+(Plasmid+%238454)/pm41915470-258-32-32
    Average 96 stars, based on 1 article reviews
    addgene plasmid - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results